The word of the day is isocratic:
(chemistry, of an HPLC system) which resolves a solute using a solvent system that does not change composition during the run. (Wiktionary)
The elution program comprised isocratic conditions with 87.5:12:0.5
(v/v/v) chloroform/methanol/buffer (1 M formic acid, neutralized to pH 3
with triethylamine) from 0 to 7 min, followed by a linear gradient from
87.5:12:0.5 (v/v/v) to 28:60:12 (v/v/v) chloroform/methanol/buffer from
7 to 27 min."
- Cristina Montealegre, Vito Verardo, Ana Gómez-Caravaca, Carmen García-Ruiz, María Luisa Marina, Maria Fiorenza Caboni, "Molecular Characterization of Phospholipids by High-Performance Liquid Chromatography Combined with an Evaporative Light Scattering Detector, High-Performance Liquid Chromatography Combined with Mass Spectrometry, and Gas Chromatography Combined with a Flame Ionization Detector in Different Oat Varieties", Journal of Agricultural and Food Chemistry 60:10963 (November 7, 2012)
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